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Treatment

Surgical Sperm Retrieval (PESA, TESA, TESE, micro-TESE)

Surgical sperm retrieval is a minor procedure that collects sperm directly from the epididymis or testes when there are no sperm in the ejaculate (azoospermia) or ejaculation is not possible. The retrieved sperm are then used with ICSI — where a single sperm is injected into each egg — to achieve fertilisation. Even a very small number of sperm can be enough. At Aansh Hospital & IVF Center — a government-registered Level-2 ART clinic (Reg. No. MH/AC/2024/15441/L2/Chandrapur/132) — retrieval is performed under anaesthesia, and sperm are processed and, where possible, frozen in our in-house lab. Discreet, couple-centred care. A diagnosis of azoospermia does not mean biological fatherhood is off the table — in many cases sperm can still be found. The retrieval is planned and overseen by Dr. Shweta Agarwal (MBBS, DGO) alongside the partner's IVF cycle, and retrieved sperm are handled and cryopreserved in our in-house lab by Senior Clinical Embryologist Aayush Agarwal, Ph.D. You can ask questions privately on WhatsApp. In Marathi and Hindi, this is शुक्राणू पुनर्प्राप्ती (sperm retrieval), part of treating पुरुष वंध्यत्व (male infertility).

Medically reviewed by Dr. Shweta Agarwal, MBBS, DGO · Last updated June 2026
Dr. Shweta Agarwal, Founder & Lead Fertility Specialist, at Aansh Hospital & IVF Center, Chandrapur Govt. ART-registered
Dr. Shweta Agarwal MBBS, DGO · Reproductive Medicine
5,000+IVF babies
30+Years of experience
4.9★500+ reviews · Google, JustDial, Practo
94%AI embryo-analysis accuracy · Garbha.ai
ART Level 2 RegisteredGovt. of India — ART Act 2021
Dr. Shweta AgarwalMBBS, DGO · Reproductive Medicine
On-site embryology labLed by Aayush Agarwal, Ph.D.
Marathi · Hindi · EnglishChandrapur · Nagpur · Vidarbha

Medically reviewed by Dr. Shweta Agarwal, MBBS, DGO. Last updated: June 2026.

Information on this page is educational and does not replace a medical consultation. Outcomes depend on individual clinical factors.


Who needs surgical sperm retrieval?

Surgical sperm retrieval is recommended when no sperm reach the ejaculate, or when ejaculation is not possible. The main indications are:

  • Obstructive azoospermia: Sperm are produced normally but a blockage stops them leaving — for example after a previous vasectomy, an infection, injury, or an absent vas deferens. Retrieval here is usually highly successful.
  • Non-obstructive azoospermia: The testes produce very little sperm. Sperm can still often be found in small pockets of tissue, particularly with the more thorough techniques.
  • Ejaculation failure: Inability to ejaculate due to spinal-cord injury or certain other conditions.
  • Prior vasectomy: Men who have had a vasectomy and now wish to conceive — retrieval with ICSI is often a more reliable route than vasectomy reversal, especially many years after the procedure.

The distinction between obstructive and non-obstructive azoospermia is important because it guides which technique is chosen and what to expect. This is established beforehand through a fertility workup — hormone tests, examination, and sometimes genetic testing. (See azoospermia and male infertility.)


What are the sperm retrieval techniques?

The technique is matched to the cause and to where sperm are most likely to be found, starting with the least invasive option. All are day-care procedures.

Technique What it involves Best suited to
PESA (Percutaneous Epididymal Sperm Aspiration) A fine needle aspirates fluid from the epididymis — no incision Obstructive azoospermia (e.g. prior vasectomy, blockage)
TESA (Testicular Sperm Aspiration) A needle aspirates tissue directly from the testis When PESA does not yield sperm; obstructive cases
TESE (Testicular Sperm Extraction) A small incision removes a tiny piece of testicular tissue, examined for sperm Non-obstructive azoospermia (production problem)
micro-TESE (Microdissection TESE) TESE performed under an operating microscope to identify the tubules most likely to contain sperm Non-obstructive azoospermia, to maximise the chance of finding sperm while removing the least tissue
  • PESA and TESA are needle-based, quick, and need no incision — recovery is rapid.
  • TESE and micro-TESE are small surgical procedures; micro-TESE uses a microscope to target sperm-bearing tubules, which can improve the chance of finding sperm in non-obstructive cases while sparing healthy tissue.

The choice is individualised — your doctor explains which technique is recommended for your situation and why before the procedure.


How does sperm retrieval pair with ICSI?

Retrieved sperm are almost always used with ICSI, not standard IVF. Because surgically retrieved sperm are fewer and often less mature than ejaculated sperm, they cannot fertilise an egg on their own in a dish — so the embryologist injects a single selected sperm directly into each egg.

The two procedures are coordinated: the female partner undergoes ovarian stimulation and egg retrieval, and the sperm retrieval is timed to the same window (or uses previously frozen retrieved sperm). Only one sperm is needed per egg, so even a handful of viable sperm can fertilise several eggs. Where extra sperm are found, they are frozen for future cycles — see sperm freezing — sparing you a repeat procedure.


What anaesthesia is used, and what is recovery like?

Sperm retrieval is performed under local anaesthesia or mild sedation, so you do not feel pain during the procedure. It is a day-care procedure — you go home the same day.

Afterwards:

  • Mild scrotal soreness or tenderness for a few days is common and managed with simple pain relief.
  • Most men return to normal activities within 2–3 days, with heavy lifting and strenuous exercise avoided for a short period.
  • A supportive garment and an ice pack help with comfort and swelling.
  • Needle-based techniques (PESA/TESA) generally have a quicker recovery than incisional ones (TESE/micro-TESE).

Your team gives you clear aftercare instructions, and you can reach us on WhatsApp with any questions during recovery.


How much does sperm retrieval cost?

The cost depends on the technique used (needle-based PESA/TESA versus surgical TESE/micro-TESE), anaesthesia, and whether sperm freezing is added. Because retrieval is paired with an IVF/ICSI cycle for the female partner, it is usually quoted as part of the overall treatment plan, with a transparent written estimate before anything begins.

Final cost depends on the technique and individual clinical evaluation — see Costs & EMI for current pricing.

  • 0% EMI options are available (3–24 months).
  • See the IVF cost & 0% EMI page for the full breakdown, including ICSI and freezing.

Good to know

Frequently asked questions

What is the difference between obstructive and non-obstructive azoospermia?
Obstructive azoospermia means sperm are produced normally but cannot exit because of a blockage in the reproductive tract. Non-obstructive azoospermia means the testes produce very little sperm. The distinction matters because it determines which retrieval technique is most appropriate and what to expect — obstructive cases generally have higher retrieval success.
Is surgical sperm retrieval painful?
No. All retrieval procedures are performed under local anaesthesia or mild sedation, so you feel no pain during the procedure. Some men have mild scrotal soreness or tenderness for a few days afterwards, easily managed with simple pain relief. Most return to normal activities within 2–3 days.
How much sperm is needed — is a small amount enough for ICSI?
Only a single healthy sperm is needed to fertilise one egg through ICSI. Even if only a few sperm are retrieved, the embryologist can use them for fertilisation. Any additional healthy sperm recovered are frozen for future use, which can avoid the need for another retrieval procedure.
What happens if no sperm are found during retrieval?
In severe non-obstructive azoospermia there is a chance no viable sperm are found, though this is less common with thorough techniques like micro-TESE. A careful diagnostic assessment beforehand helps estimate the likelihood of success. If no sperm are found, Dr. Shweta Agarwal will discuss your options sensitively and privately.
Can retrieved sperm be frozen for later cycles?
Yes. When more sperm are retrieved than needed for the immediate cycle, they are cryopreserved through sperm freezing. This is especially valuable in azoospermia, as future IVF/ICSI cycles can then use the frozen sperm without another surgical retrieval. How many cycles this supports depends on how many viable sperm are found.
I had a vasectomy years ago — can I still father a child?
Often, yes. Sperm retrieval (typically PESA or TESA) combined with ICSI is a reliable route to conception after a vasectomy, and is frequently preferred over vasectomy reversal — particularly many years later, when reversal success declines. Retrieval works regardless of how long ago the vasectomy was performed.
Do I need a genetic test before retrieval?
For men with very low counts or azoospermia, genetic testing (such as karyotype and Y-chromosome microdeletion analysis) is often recommended. It helps estimate the chance of finding sperm and identifies any genetic condition that could be relevant to treatment and to children. This information supports fully informed decisions before the procedure.
Will the procedure and consultation stay confidential?
Yes. The procedure and all consultations are kept private, and sperm are handled in our in-house lab. The male retrieval is coordinated with the partner's IVF cycle as part of the couple's care, and you can ask questions discreetly on WhatsApp at any stage.
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